Identification of an NAD-dependent 3-acetamidobenzamide-sensitive system in oncospheres from the tapeworm Hymenolepis diminuta.

( a ) Autoradiograph of RNA species which arise in a transcription reaction. Nomenclature, fragment sizes and experimental conditions are as in 141. RNA circles are arrowed wth their tentative sizes in nucleotides. Precursor (p), ribozyme (IVS), ligated exons (SE3E) and precursor in which the downstream exon has been hydrolysed (5EI) are depicted. ( b ) Autoradiograph of radiolabelled amplified DNA of the material used for electrophoresis in ( u ) . Three doublets arising from DNA fragments of 108, 1 I2 and 127 bp arc observed. The left panel shows the four tracks of an M 13 DNA sequence used as a size marker; the cross denotes nucleotide position I27 from the 5' end of the universal primer.